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- In-Vitro Laboratory Research Use Only
5-Amino-1MQ (5-Amino-1-methylquinolinium) is a small-molecule research compound utilized as a selective chemical probe for investigating the enzymatic activity of Nicotinamide N-methyltransferase (NNMT). It is primarily employed in metabolic systems biology to examine the interactions between nicotinamide metabolism, methyl-donor utilization, and NAD⁺-dependent signaling pathways. This compound is supplied exclusively for in-vitro laboratory research and development use.
In metabolic research models, 5-Amino-1MQ is used to disrupt the methyl-group “sink” created by elevated NNMT activity.
Adipocyte Metabolism
Studies investigate lipogenesis and insulin-related signaling in white adipose tissue (WAT) explants and cellular systems.
NAD⁺ Flux Analysis
Research measures intracellular NAD⁺ concentrations and downstream activation of NAD⁺-dependent enzymes, including sirtuins, following NNMT inhibition.
Epigenetic Modulation
Experimental systems examine how altering the S-adenosylmethionine (SAM) to S-adenosylhomocysteine (SAH) ratio impacts histone methylation patterns and chromatin-associated signaling.
Elevated NNMT expression is a hallmark of several cancer-associated cell types, making 5-Amino-1MQ a useful probe in mechanistic oncology research.
Tumor Microenvironment
Studies investigate metabolic reprogramming in cancer-associated fibroblasts (CAFs) and associated signaling effects.
Membrane Fluidity & Signaling
Research explores how altered methylation potential influences membrane composition and receptor density.
Proliferation Assays
In-vitro models assess the impact of NNMT inhibition on cell-cycle progression in NNMT-overexpressing cell lines.
Pathway Complexity
While 5-Amino-1MQ targets NNMT directly, downstream effects on one-carbon metabolism and methyl-donor availability are complex and context-dependent.
Model Specificity
Observed effects vary based on baseline NNMT expression, which differs significantly across tissues (e.g., liver vs. adipose).
Research Grade Only
This compound is a chemical probe intended solely for defining biological mechanisms in non-human experimental models and is not formulated for therapeutic administration.
Research has evaluated specificity against related methyltransferases and NAD⁺ salvage enzymes, including:
(Selectivity profiles are assay-dependent and may vary across experimental conditions.)
Neelakantan, H., et al. (2018).
Small molecule nicotinamide N-methyltransferase inhibitor activates senescent muscle stem cells and improves regenerative capacity of aged skeletal muscle.
Biochemical Pharmacology.
Liu, J. R., et al. (2021).
Nicotinamide N-methyltransferase (NNMT): A potential therapeutic target for obesity and type 2 diabetes.
Journal of Biomedical Science.
Sun, W. D., et al. (2020).
NNMT expression is associated with tumor aggressiveness and poor prognosis in gastric cancer.
Pathology – Research and Practice.
Awosemo, O., et al. (2021).
Inhibition of Nicotinamide N-methyltransferase (NNMT) by 5-amino-1-methylquinoline reduces cell growth and migration in renal cell carcinoma.
International Urology and Nephrology.
